ThermonucleotideBLAST is a software program for searching a target
database of nucleic acid sequences using an assay specific query that
is based on biochemical assays (i.e. a pair of oligonucleotide
sequences representing PCR primers or Padlock probes, a triplet of
oligos representing PCR primers and a TaqMan probe, or a single oligo
representing a hybridization probe). Unlike existing programs (i.e.
BLAST) which use heuristic measures of sequence similarity for
identifying matches between a query and target sequence,
ThermonucleotideBLAST uses free energy and melting temperature.
