Kinoshita Hiroyuki
hkino****@cat*****
2005年 10月 3日 (月) 12:24:50 JST
昨日に引き続き、もう１つ質問をさせて下さい。 emmaにてマルチプルアライメントを試したいのですが、以下のようになってしまいま した。 ClustalWが見つからないとのことですが、どのようにすればよいのでしょうか？ 長くなってしまいますが、hoxlab.fastaの内容をcatで表示させたものといっしょに 貼り付けておきます。 どなたかご教示いただきたく思います。 木下博之 knoppix ＠ ttyp1[knoppix]$ emma Multiple alignment program - interface to ClustalW program Input sequence(s): hoxlab.fasta Output sequence [scr_drome.aln]: Dendogram output filename [scr_drome.dnd]: EMBOSS An error in ajsys.c at line 398: cannot find program 'clustalw' knoppix ＠ ttyp1[knoppix]$ cat hoxlab.fasta >gi|68067436|sp|P09077|SCR_DROME Homeotic Sex combs reduced protein MDPDCFAMSSYQFVNSLASCYPQQMNPQQNHPGAGNSSAGGSGGGAGGSGGVVPSGGTNGGQGSAGAATP GANDYFPAAAAYTPNLYPNTPQAHYANQAAYGGQGNPDMVDYTQLQPQRLLLQQQQQQQQQQHAHAAAAV AAQQQQQLAQQQHPQQQQQQQQANISCKYANDPVTPGGSGGGGVSGSNNNNNSANSNNNNSQSLASPQDL STRDISPKLSPSSVVESVARSLNKGVLGGSLAAAAAAAGLNNNHSGSGVSGGPGNVNVPMHSPGGGDSDS ESDSGNEAGSSQNSGNGKKNPPQIYPWMKRVHLGTSTVNANGETKRQRTSYTRYQTLELEKEFHFNRYLT RRRRIEIAHALCLTERQIKIWFQNRRMKWKKEHKMASMNIVPYHMGPYGHPYHQFDIHPSQFAHLSA >gi|68067431|sp|P07548|DFD_DROME Homeotic deformed protein MSSFLMGYPHAPHHVQSPMSMGNGLDPKFPPLADDYHHYNGHYSMTASTGHMSGAVGGGAGVGSVGGGGA GGMTGHPHSMHPADMVSDYMAHHHNPHSHSHSHTHSLPHHHSNSAISGHHQASAGGYSSNYANATPPSHP HSHPHAHPHQSLGYYVHHAPEFISAGAVHSDPTNGYGPAANVPNTSNGGGGGGSGAVLGGGAVGGSANGY YGGYGGGYGTANGSVGSTHSQGHSPHSQMMDLPLQCSSTEPPTNTALGLQELGLKLEKRIEEAVPAGQQL QELGMRLRCDDMGSENDDMSEEDRLMLDRSPDELGSNDNDDDLGDSDSDEDLMAETTDGERIIYPWMKKI HVAGVANGSYQPGMEPKRQRTAYTRHQILELEKEFHYNRYLTRRRRIEIAHTLVLSERQIKIWFQNRRMK WKKDNKLPNTKNVRKKTVDANGNPTPVAKKPTKRAASKKQQQAQQQQQSQQQQTQQTPVMNECIRSDSLE SIGDVSSSLGNPPYIPAAPETTSSYPGSQQHLSNNNNNGSGNNNNNNNNNNSNLNNNNNNNQMGHTNLHG HLQQQQSDLMTNLQLHIKQDYDLTAL >gi|6166216|sp|P49639|HXA1_HUMAN Homeobox protein Hox-A1 (Hox-1F) MDNARMNSFLEYPILSSGDSGTCSARAYPSDHRITTFQSCAVSANSCGGDDRFLVGRGVQIGSPHHHHHH HHHHPQPATYQTSGNLGVSYSHSSCGPSYGSQNFSAPYSPYALNQEADVSGGYPQCAPAVYSGNLSSPMV QHHHHHQGYAGGAVGSPQYIHHSYGQEHQSLALATYNNSLSPLHASHQEACRSPASETSSPAQTFDWMKV KRNPPKTGKVGEYGYLGQPNAVRTNFTTKQLTELEKEFHFNKYLTRARRVEIAASLQLNETQVKIWFQNR RMKQKKREKEGLLPISPATPPGNDEKAEESSEKSSSSPCVPSPGSSTSDTLTTSH >gi|123353|sp|P09022|HXA1_MOUSE Homeobox protein Hox-A1 (Hox-1.6) (Homeotic protein ERA-1-993) (Early retinoic acid 1) (Homeoboxless protein ERA-1-399) MNSFLEYPILGSGDSGTCSARAYPSDHGITTFQSCAVSANSCGGDDRFLVGRGVQISSPHHHHHHHHHHH PQTATYQTSGNLGISYSHSSCGPSYGAQNFSAPYGPYGLNQEADVSGGYPPCAPAVYSGNLSTPMVQHHH HHQGYAGGTVGSPQYIHHSYGQEQQTLALATYNNSLSPLHASHQEACRSPASETSSPAQTFDWMKVKRNP PKTGKVGEYGYVGQPNAVRTNFTTKQLTELEKEFHFNKYLTRARRVEIAASLQLNETQVKIWFQNRRMKQ KKREKEGLLPISPATPPGSDEKTEESSEKSSPSPSAPSPASSTSDTLTTSH >gi|6226632|sp|P17488|HM13_CAEEL Homeobox protein ceh-13 MSSTECYGAPPHNYYQDWPTTHSYYPSVPSSYSPLNHHPADIWAAHPSNYIMGNGHVSPPATASGLSPPA SRSSNSSAELPTGVTASQHNTYKWMHTKRSQRPAAPKKKVIDENGTNRTNFTTHQLTELEKEFHTAKYVN RTRRTEIASNLKLQEAQVKIWFQNRRMKEKKREKEKAFLARNTWESNSPTSSCSGEDVKNFK >gi|232263|sp|Q01822|HXD1_MOUSE Homeobox protein Hox-D1 (Hox-4.9) MSSYLEYVSCAAGGGSGGVGGDVLAFAPKFCADARPVALQPAFPLGNGDGAFVSCLPLATARPTPSPPAG PAQSPVPQPAAPRYAPCTLEGAYERGAAPASAAEYGFLGSGPAFDFPGALGRAADEGGAHVHYATSAVFS GGGSFLLSGQVDFAAFGEPGPFPACLKEPADGHPGPFQTVSPAPGACPKPASPTSSLPAAHSTFEWMKVK RNAPKKSKLSEYGATSPPSAIRTNFSTKQLTELEKEFHFNKYLTRARRIEIANCLQLNDTQVKIWFQNRR MKQKKREREGLLATAASVASIKLPRSETSPIKSGRNLGSPSQAQEPS >gi|123272|sp|P17919|HXB1_MOUSE Homeobox protein Hox-B1 (Hox-2.9) MDYNRMSSFLEYPLCNRGPSAYSAPTSFPPCSAPAVDSYAGESRYGGGLPSSALQQNSGYPVQQPPSSLG VSFPSPAPSGYAPAACNPSYGPSQYYSVGQSEGDGSYFHPSSYGAQLGGLPDSYGAGGVGSGPYPPPQPP YGTEQTATFASAYDLLSEDKESPCSSEPSTLTPRTFDWMKVKRNPPKTAKVSELGLGAPGGLRTNFTTRQ LTELEKEFHFNKYLSRARRVEIAATLELNETQVKIWFQNRRMKQKKREREGGRMPAGPPGCPKEAAGDAS DQSACTSPEASPSSITS >gi|123271|sp|P14653|HXB1_HUMAN Homeobox protein Hox-B1 (Hox-2I) MDYNRMNSFLEYPLCNRGPSAYSAHSAPTSFPPSSAQAVDSYASEGRYGGGLSSPAFQQNSGYPAQQPPS TLGVPFPSSAPSGYAPAACSPSYGPSQYYPLGQSEGDGGYFHPSSYGAQLGGLSDGYGAGGAGPGPYPPQ HPPYGNEQTASFAPAYADLLSEDKETPCPSEPNTPTARTFDWMKVKRNPPKTAKVSEPGLGSPSGLRTNF TTRQLTELEKEFHFNKYLSRARRVEIAATLELNETQVKIWFQNRRMKQKKREREGGRVPPAPPGCPKEAA GDASDQSTCTSPEASPSSVTS >gi|833615|emb|CAA48418.1| Ghox-lab [Gallus gallus] MDNTRMNSFLEYAICNRGTGAYHHLEQSSPSFPSCSGSPSSDTFHGEGAYGAGGSPVAAAHLPPQSPGFF PAPPGAPSLRGRCPERRVPNPKLQPRLRHHQLYLGQQDADAVYFQAAGYSSSTGPHLGSLAEGYCGAVGQ YQQQQHLYGQEQPSYLPGVYGNLSPSLNEDKDPACPSEPCPNASRARTFDWMKVKRNPPKTAKVSEYGLL GQPNTIRTNFTTKQLTELEKEFHFNKYLTRARRVEIAATLELNETQVKIWFQNRRMKQKKREKEGLAPPA ASRSAKEASEASDQSNCTSPEASPSSVSS >gi|60392416|sp|Q98SI1|HXA1A_BRARE Homeobox protein Hox-A1a (Hox-A1) MSTFLDFSSISGGGDGGSGGSCSVRAFHGDHGLSTFQSSCAVRLNSCSGDERFMSNISSQDVINSQPQQA GSYQSPGTLSITYSAHPSYGTQSFCTGYNHYALNQDVESSVSFPQCGPLVYSGNISSTVVQHRHHRHGYS SGNVHLHGQFQYGSATYGNSSDQANLTFVAGCSNPLSPLHVPHHDACCSPLSDGVPTGQTFDWMKVKRNP PKTGKAGEYGFGGQPNTVRTNFSTKQLTELEKEFHFNKYLTRARRVEIAASLQLNETQVKIWFQNRRMKQ KKREKEGLLPKSLSEQKDGLEKTEDASEKSPSAPSTPSPSPTVEAYSSN >gi|60392415|sp|Q90423|HXB1B_BRARE Homeobox protein Hox-B1b (Hox-A1) MNSYLDYTIYNRGSNTYSSKVGCFPVEQEYLPSACASTNSYIPEGRPVGGNTFTSAPHETHGTSYAQIQS QPFHLNVDMGKTGHSNFCKQTRPPHSDYGHQHVLTQADDHMRLQSPGFSVVNMGANIGTYSESNCRPGSV SASHYQSYAYGEPEPHGCGSFSKYQVSPDSDSDSKTNIKQAPTFDWMKVKRNPPKTVKVAEYGIHGQQNI IRTNFTTKQLTELEKEFHFNKYLTRARRVEVAATLELNETQVKIWFQNRRMKQKKREKEGTAPVIKRVTL CSSGQNADHSTSSSPGASPTSDSSTAI >gi|60392402|sp|O42366|HXB1A_BRARE Homeobox protein Hox-B1a (Hox-B1) MNSFLEYTICNRGTNAYSPKAGYHHLDQAFPGPFHTGHASDSYNADGRLYVGGSNQPPTAAAQHQHQNGI YAHHQHQNQTGMGLTYGGTGTTSYGTQACANSDYAQHQYFINPEQDGMYYHSSGFSTSNASPHYGSMAGA YCGAQGAVPAAPYQHHGCEGQDHQRAYSQGTYADLSASQGTEKDTDQPPPGKTFDWMKVKRNPPKTGKVA EYGLGPQNTIRTNFTTKQLTELEKEFHFSKYLTRARRVEIAATLELNETQVKIWFQNRRMKQKKREKEGL APASSTSSKDLEDQSDHSTSTSPEASPSPDS >gi|17378648|sp|Q9GZZ0|HXD1_HUMAN Homeobox protein Hox-D1 MSSYLEYVSCSSSGGVGGDVLSLAPKFCRSDARPVALQPAFPLGNGDGAFVSCLPLAAARPSPSPPAAPA RPSVPPPAAPQYAQCTLEGAYEPGAAPAAAAGGADYGFLGSGPAYDFPGVLGRAADDGGSHVHYATSAVF SGGGSFLLSGQVDYAAFGEPGPFPACLKASADGHPGAFQTASPAPGTYPKSVSPASGLPAAFSTFEWMKV KRNASKKGKLAEYGAASPSSAIRTNFSTKQLTELEKEFHFNKYLTRARRIEIANCLHLNDTQVKIWFQNR RMKQKKREREGLLATAIPVAPLQLPLSGTTPTKFIKNPGSPSQSQEPS >gi|14916591|sp|Q9IA19|HXA1_HETFR Homeobox protein Hox-A1 MDNARMNSFLDYSIINGETGTCSSRSYHADQGITTYQSCAVSNNNCNADDRYIVSRSVQIGAPPPHHHHH QSNYTHPNNLGISYSAHPNCGAGYPAQSFNTGYSHHYSLNQETDGNGGYPQCAPAVYPGNIASAISPHHH SYGGMVGSGQYPHHPYGQEQQGLALAAGCHSLSPVHGSHQEACCSPSAETPPPAQTFDWMKVKRNPPKTG KAGEYGFAGQPNTVRTNFTTKQLTELEKEFHFNKYLTRARRVEIAAALQLNETQVKIWFQNRRMKQKKRE KEGLTSASPATPGSEANTEDTSDKCNSTSSTPSPSSSTSETINTSG >gi|6016290|sp|O08656|HXA1_RAT Homeobox protein Hox-A1 MDNARLNSFLEYPILGSGDSGTCSARVYSSDHGITTFQSCAVSANSCGGDDRFLGGRGVQITSPHHHHHH HHHPQPATYQTSGNLGVSYSHSSCGPSYGAQNFSAPYGPYGLNQEADVSGGYPPCAPAVYSGNLSSPMVQ HHHHHQGYAGGTVGSPQYIHHSYGQEHQSLALATYNNSLSPLHASHQEACRSPASETSSPAQTFDWMKVK RNPPKTGKVGEYGYVGQPNAVRTNFTTKQLTELEKEFHFNKYLTRARSEIAASLQLNETQVKIWFQNRRM KQKKREKEGLLPMSPATPPGSDEKTEESSEKSSSSPSAPSPASSTSDTLTTSH >gi|2495327|sp|Q08820|HXD1_XENLA Homeobox protein Hox-D1 (Hox.lab1) MNSYLEYTSCGDVLAFSPKFCRSDHRNMNLQPYPRSGADHPFMPVGGVPGSVAHQAPHQSPALYAPCSLD VAYEPTPPSDYSFLHSSPDYDYYGSNHQIEETGGHIPYGSSLFPGNGSYILNGQLSYRTLGEETQMAQCK EPLEVYPGGNLQSISPSPGTYPKPASPASDSYVSTFDWMKVKRNPPKKSIPSEYGVTSPPCNVRTNFTTK QLTELEKEFHFNKYLTRARRIEIANSLQLNDTQVKIWFQNRRMKQKKREREGTLPNSPPSGPASNFCVKP PHLKSVNETATLSPSKDASP >gi|2495323|sp|Q90346|HXB1_CYPCA Homeobox protein Hox-B1 MDNSSMNSFLEYTICNRGTNAYSPKAGYQHLDQALSGPFHTGHARYSHNADGRLYIGGSNQPAAAQHQHQ SGVYAHHQHQTHQSGIGLTYGGTGTTSYGTQACANPDYAQHQYFINPEQDGMYYHSSGFSNSNLGPHYGS MAGAYCGAQGAVPAAPYQHHGCEGQDHQRGYSQGTYADLSRSQGRERDTDQSPPGKTFDWMKVKRNPPKT AKVADYGLGPQNTIRTNFTTKQLTELEKEFHFSKYLTRARRVEIAATLELNETQVKIWFQNRRMKQKKRE KEGLAPASSTLSKDLEDHSDHSTSTSPGASPSPDS >gi|399997|sp|P31259|HXB1_CHICK Homeobox protein Hox-B1 (Ghox-lab) MDNTRMNSFLEYAICNRGTGAYHHLEQSSPSFPSCSGSPSSDTFHGEGAYGAGGSPVAAAHLPPQSPGFF PAPPGAPSLRGRCPERRVPNPKLQPRLRHHQLYLGQQDADAVYFQAAGYSSSTGPHLGSLAEGYCGAVGQ YQQQQHLYGQEQPSYLPGVYGNLSPSLNEDKDPACPSEPCPNASRARTFDWMKVKRNPPKTAKVSEYGLL GQPNTIRTNFTTKQLTELEKEFHFNKYLTRARRVEIAATLELNETQVKIWFQNRRMKQKKREKEGLAPPA ASRSAKEASEASDQSNCTSPEASPSSVSS
